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Haemoglobin F and A2 profiles among sickle cell anaemia patients in Lagos State University Teaching Hospital (LASUTH), Nigeria
Akinsegun Akinbami1, Ebele Uche1, Adedoyin Dosunmu1, Bodunrin Osikomaiya2, Adewumi Adediran3, John-Olabode Sarah3, Oluwole Esther4, Mulikat Badiru5, Rafat Bamiro5
1 Department of Hematology and Blood Transfusion, Lagos State University, College of Medicine, Ikeja, Lagos, Nigeria
2 Department of Haematology and Blood Transfusion, Gbagada General Hospital, Lagos, Nigeria
3 Department of Haematology and Blood Transfusion, College of Medicine, University of Lagos, Lagos, Nigeria
4 Department of Community Health and Primary Health Care, College of Medicine, University of Lagos, Idiaraba, Lagos, Nigeria
5 Department of Haematology and Blood Transfusion, Lagos State University Teaching Hospital, Ikeja, Lagos, Nigeria
Correspondence Address:
Dr. Akinsegun Akinbami
Department of Hematology and Blood Transfusion, Lagos State University, College of Medicine, PMB 21266, Ikeja, Lagos, Lagos State
Nigeria
 Source of Support: None, Conflict of Interest: None  | 2 |
DOI: 10.4103/atp.atp_45_17
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Background: The choice of high-performance liquid chromatography (HPLC) to measure HbF and HbA2in sickle cell disease patients is regarded as a method of choice by many researchers. This study was aimed at using HPLC in determining the mean and gender-specific reference values of HbF and HbA2in sickle cell anemia (SCA) population and bringing to fore all associated implications. Materials and Methods: This was a cross-sectional, retrospective, descriptive study involving SCA patients. All case notes containing HPLC hemoglobin quantification reports were reviewed to extract the percentages of HbA2, HbF, and HbS of patients. The demographic data of individual patients were also obtained from the records. Data were analyzed with IBM SPSS Statistics for Windows, Version 20.0 Armonk, New York, USA. Results: A total of 100 participants' records were reviewed consisting of 40 (40%) males and 60 (60%) females. The overall mean age (±standard deviation [SD]) of participants was 25.89 years ±9.34. The overall mean HbF and HbA2were 6.94% ±5.05 and 3.75% ±0.74, respectively. Thirty percent had HbF <3%, whereas 34% of them had elevated HbA2level >4%. The mean (±SD) HbF and HbA2for both males and females were 6.97% ±5.45 and 3.68% ±0.58, 6.92% ±4.87, and 3.80% ±0.83, respectively. Conclusions: Thirty percent of the study participants had HbF <3%, whereas 34% of them had elevated HbA2level >4% and could indeed be carrying beta thalassemia trait with the sickle cell gene. |
