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ORIGINAL ARTICLE
Year : 2016  |  Volume : 7  |  Issue : 2  |  Page : 112-116

Immunohistochemical analysis of human papilloma virus on cervical biopsy in patients attending Aminu Kano Teaching Hospital, Kano


1 Medical Laboratory Science Department, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria
2 Anatomy Department, Faculty of Basic Medical Sciences, University of Benin, Benin City, Nigeria
3 Pathology Department, College of Health Sciences, Bayero University, Kano, Nigeria

Correspondence Address:
G Y Riruwai
Medical Laboratory Science Department, Faculty of Allied Health Sciences, Bayero University, Kano
Nigeria
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Source of Support: None, Conflict of Interest: None


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Background: Epidemiological study has shown that human papilloma virus infections play a major role in cervical pre-invasive and invasive lesions. Aim: Application of immunological methods can detect Human Papilloma Virus genetic material in almost 100% of the premalignant and malignant tissues of the uterine cervix. Materials and Methods: One hundred and twenty five blocks previously prepared cervical biopsy block from January, 2015 to December, 2015 were retrieved and re-evaluated to confirm the nature of specimen microscopically. Clinical data such as the age, sex and routine diagnosis were extracted from the record in the Histopathology Department of Aminu Kano Teaching Hospital Kano. Result: Statistically one hundred and twenty five blocks were identified and used for the study. Out of which, 32(25.6%) were age group 20-30 years, 32(25.6%) were 31-40 years, 29(23.2%) were 41-50 years and 32(25.6%) were 50 years and above. Relationship of age and different stages of dysplastic lesions shows women of 20-30 years have 2(6.3%) of inflammation, 1(3.1%) CIN1, 4(12.5%) CIN2, 0(0%) CIN3 and 25(78.1%) are cancer. Thirty one to fourty years were 0 (0%) inflammations, 5(15.6%) CIN1, 1(3.1%) CIN2, 3(9.4%) CIN3, 25(78.1%) were cancers. Fourty one to fifty years has 0 (0%) inflammations, 5(15.6%) CIN1, 1(3.1%) CIN1, 3(9.4%) CIN3, 23(71.9%) are cancers. Fifty one years and above were found to have no inflammation, CIN1, CIN2, and CIN3 while 32(100%) were cancers. This indicate that (p<0.01.) using Anova Statistical package. Conclusion: Immunological staining using P16 can be used to differentiate High Risk Human Papilloma Virus from Low Risk Human Papilloma Virus in cervical lesions. P16 antibody is found to be useful as a dysplasia associated antigen in differentiating High Risk Human Papilloma Virus from Low Risk Human Papilloma Virus.


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